Antibodies specific for CDK-11.2 were prepared by YenZym Antibodies LLC (San Francisco, CA). Rabbits were immunized with a peptide whose sequence corresponded to amino acids 626-642 of CDK-11.2: CPENRRKNRLEALLADEE. cdk-11.2
CSC-1 and BUB-1 antibodies were generated by Covance in rabbits using recombinant GST-BUB-1 (amino acids 287-661) and GST-CSC-1 (full length) proteins. Serum was then affinity purified and antibodies were used at 1:1500 (BUB-1) and 1:1000 (CSC-1). csc-1
CSC-1 and BUB-1 antibodies were generated by Covance in rabbits using recombinant GST-BUB-1 (amino acids 287-661) and GST-CSC-1 (full length) proteins. Serum was then affinity purified and antibodies were used at 1:1500 (BUB-1) and 1:1000 (CSC-1). bub-1