Rabbit polyclonal antibodies to C. elegans HSF-1 were generated using purified, recombinant C. elegans HSF-1, which was recovered from E. coli expressing His6-HSF-1 from a modified version of the pET-28a(+) vector by affinity purification and subsequent on column thrombin cleavage to remove the His6 tag. Polyclonal rabbit antibodies to the full-length, untagged protein were generated by Covance.
hsf-1