To generate pHTP-1(S325) phosphospecific antibody, phospho peptide (AQM[pS]PIQ) was synthesized and highperformance liquid chromatography fractionated (CPC Scientific Inc., CA). A Cterminal CysLysLysLys was added to facilitate maleimidebased conjugation of peptides to KLH for immunization and to bovine serum albumin for purification of the antibodies. Sera were gener ated from immunized rabbits by Yenzym Antibodies, LLC (CA).
htp-1