The primary antibody against ATP5B (which detects C. elegans ATP-2) was custom made and suitable for the analysis of complexes using native PAGE (gift from Peter Rehling). atp-2
A polyclonal antibody against a SYP-1 peptide (VDAPTEALIETPVDDQSSGFLC) was generated by the GenScript Antibody Group and used at a 1:3000 dilution for immunofluorescence and 1:5000 dilution for western blot analysis. syp-1
Polyclonal antibody against MEF-2 recombinant protein. The polyclonal antibodies recognized the fusion protein by Western blot analysis, but unable to detect the endogenous CeMEF2 protein by either Western blot or in situ immunolocalization. mef-2
Antibodies were raised against the tandem repeat region of ANC-1; the specificity of the affinity-purified antibody was shown by immunoblot analysis and by the fact that purified antibodies in anc-1 mutant tissues failed to localize. anc-1
A synthetic peptide corresponding to amino acids 2-24 of SYP-1 protein (DNFTIWVDAPTEALIETPVDDQS) was used to generate anti-SYP-1 polyclonal antibodies in rabbits (Genscript). Raw sera were affinity purified and employed for immunostaining analysis. syp-1
Rabbit polyclonal antibodies raised against a UBC-2 fusion protein expressed in E.coli. The anti-UBC-2 antiserum was affinity purified on a UBC-2 column, and the specificity of the affinity-purified antibodies was verified bu Western blot analysis. let-70
Rabbit polyclonal antibody was raised against a maltose binding protein-TLK-1 fusion protein (MBP-TLK-1). Western blot analysis of wild-type C. elegans embryo lysates probed with affinity-purified TLK-1 antisera revealed asingle protein of the predicted size (108 kDa). Antibody recognition of this protein was entirely eliminated in lysates from tlk-1(RNAi) embryos. tlk-1