antibodies were generated against recombinant human SMG-1 (amino acids 1827-1926), SMG-8 (amino acids 563-771), or SMG-9 (amino acids 21-75) proteins fused to GST. smg-1
BL52/B6 mice were subcutaneously primed with 100 μg FIC-1 in PBS supplemented with complete Freund's adjuvant, boosted by intraperiotenal injection 4 weeks later with 20 μg in PBS and terminally bled 5 days later. fic-1