cpl-1 (NM_ 001269789) gene was amplified by PCR and constructed into the prokaryotic expression plasmid (pET28a). Then, the recombinant protein was expressed in the BL21 E. coli expression system and purified. After that, the recombinant protein was injected into the rabbit, and the serum was collected. Finally, we detected the titer at 1:256,000 using enzyme-linked immunosorbent assay and identified good specificity using western blot.
cpl-1