An anti-IFC-2 antibody was prepared in guinea pig using recombinant IFC-2e. For the expression of IFC-2e in E. coli bacteria, we cloned the coding sequence of ifc-2e into the NheI/BamHI sites of plasmid pET24a(+) (Merck, Darmstadt, Germany, #69749) giving rise to plasmid pIFC-2e (#1856)
ifc-2