The synthetic peptide HIS-24-K14me1 (N-CSDSAVVAAAVEP-K(me)-VPKAKAAK-C) was chemically synthesized and coupled with sulfo-maleimidobenzoyl-N- hydroxysuccinimide ester (Squarix biotechnology GmbH, Marl, Germany) to bovine serum albumin. 500 mg of antigen was used for immunization of three rabbits in a series of three injections. Antigen injections and resulting antiserum collections were performed by Charles River.
his-24