WBAntibody00002847
The murine monoclonal against phosphorylated S58 of FEZ1 was generated following the standard protocol from Synaptic Systems (Gttingen, Germany). Briefly, three 8- to 10-week-old female BALB/c mice were immunized over a period of 17 days with an FEZ1 phospho-peptide (EIISFKpSMEDL) coupled to KLH. Cells from knee lymph nodes were fused with the mouse myeloma cell line P3 63Ag.653 (ATCC CRL-1580). Cell culture supernatants obtained from individual clones were then screened using an enzyme-linked immunosorbent assay (ELISA) and immunoblot assays to ascertain their speci city towards phospho-58. e nal hybridoma used in this study was cloned two times by limiting dilution. e monoclonal antibody produced from this clone was determined to be of the IgG2a subclass.
WBAntibody00002844
CSC-1 and BUB-1 antibodies were generated by Covance in rabbits using recombinant GST-BUB-1 (amino acids 287-661) and GST-CSC-1 (full length) proteins. Serum was then affinity purified and antibodies were used at 1:1500 (BUB-1) and 1:1000 (CSC-1).
csc-1 WBAntibody00002845
CSC-1 and BUB-1 antibodies were generated by Covance in rabbits using recombinant GST-BUB-1 (amino acids 287-661) and GST-CSC-1 (full length) proteins. Serum was then affinity purified and antibodies were used at 1:1500 (BUB-1) and 1:1000 (CSC-1).
bub-1 WBAntibody00003000
α-SYP-1 (1:500, VDAPTEALIETPVDDQSSGFL)
syp-1 WBAntibody00001789
Antibody against CCT-1/TCP-1.
cct-1 WBAntibody00002992
Anti-TIM-1 [antigen 1-222 sheep]
tim-1 WBAntibody00002995
Anti-LRR-1 [antigen 1-222 sheep]
lrr-1 WBAntibody00002993
Anti-TIPN-1 [antigen 1-222 sheep]
tipn-1 WBAntibody00002990
Anti-CLSP-1 [antigen 1-222 sheep]
clsp-1 WBAntibody00000634
Antibody against SMC-1/HIM-1 recombinant protein.
him-1