Antibodies against CYK-4 were generated by injecting a purified GST fusion with CYK-4 aa 131-345 into rabbits (Covance). Antibodies were purified from serum using standard procedures (Harlow, 1988) on a 1 ml NHS HiTrap column (GE Healthcare Life Sciences) containing an immobilized MBP fusion with the same region of CYK-4.
cyk-4