The monoclonal antibody against C. elegans DLG-1 was made by immunizing mice with a His6-tagged fusion protein encompassing amino acids 204-593 (PDZ domain 1-3). This construct contained the same DLG-1 coding fragment that had previously been reported for the generation of rabbit and rat polyclonal antibodies. One stable hybridoma cell line, 5E2, was produced and isotyped as IgG1.
dlg-1