To generate the mouse monoclonal anti-PARG-1(2D4) antibody, the cDNA encoding for residues 1-350 of C. elegans PARG-1 (isoform A) was generated by gene synthesis (IDT) and then cloned into pCoofy31 in frame with a C-ter 6 × His tail. The resulting plasmid was expressed in E. coli BL21 cells according to standard procedures and 1 mg of purified protein was used to immunize three mice in the 'in-house' monoclonal antibody facility at Max Perutz Laboratories.
parg-1