Polyclonal rabbit antibody. A 350-bp (Glu-69 to Leu-180 of VHA-6) cDNA fragment was inserted into pGEX5X-1(Amersham Pharmacia Biotech). The recombinant proteins expressed in Escherichia coli were used to immunize rabbits, and the antisera were affinity-purified using the same recombinant protein.
vha-6