Antisera to CAND-1 was produced in rabbits by immunization with a fusion protein expressed in E. coli comprising the C-terminal 374 amino acids of CAND-1 linked to a His tag in the pET15b vector. cand-1
The rabbit anti-GLD-2 antibody used in the immunoprecipitation experiments was generated by immunizing New Zealand White rabbits with an epitope that covered amino acid 959 to 1113 of GLD-2, fused to a GST-affinity tag. gld-2