A polyclonal rabbit antibody to TIMM-23 was generated against the synthesized polypeptide of amino acids 95-230 and affinity- purified (ABClonal Science). timm-23
Fragment of PGL-1 (95-551) was cloned into the pET-28a vector, expressed as His-tagged fusion proteins in E. coli BL21 and purified for use as an immunogen in rabbits (for PGL-1). pgl-1
Antisera to CAND-1 was produced in rabbits by immunization with a fusion protein expressed in E. coli comprising the C-terminal 374 amino acids of CAND-1 linked to a His tag in the pET15b vector. cand-1
The rabbit anti-GLD-2 antibody used in the immunoprecipitation experiments was generated by immunizing New Zealand White rabbits with an epitope that covered amino acid 959 to 1113 of GLD-2, fused to a GST-affinity tag. gld-2
Purified UNC-45 His6-tag-fusion protein was used to generate two anti-UNC-45 polyclonal antisera in rabbits (BioGenes). The anti- serum was purified by affinity-chromatography and tested for specificity by immunodetecion of UNC-45 using C. elegans total worm extract and recombinant UNC-45. unc-45