EMB-9::mcherry strongly localized to BM in pharynx. We also confirmed strong expression of EMB-9::mcherry in coelomocytes, but not in the strain expressing mkate2::NID-1. The mkate2::NID-1 and EMB-9::mcherry are similarly localized to BM at gonad and uterus.
Consistent with previous studies, the type-IV collagen α1 chain construct, EMB-9::mCherry, when expressed from the integrated transgene tkTi1[emb-9::mCherry], localized to the gonadal basement membrane in WT C. elegans.
Both MIG-17 and EMB-9 localize in the head-region to a pattern reminiscent of the extracellular matrix proximal to the pharynx bulb. EMB-9 protein levels increased as animals progress through the larval stages, achieving maximal expression in the adult stage.
mkate2::NID-1strongly localized to intestinal BM and localized to gonadal BM and nerve ring, consistent with previous reports. Interestingly, we found that EMB-9::mcherry strongly localized to BM in pharynx, although mkate2::NID-1 was faintly localized at pharyngeal BM. We also confirmed strong expression of EMB-9::mcherry in coelomocytes, but not in the strain expressing mkate2::NID-1. The mkate2::NID-1 and EMB-9::mcherry are similarly localized to BM at gonad and uterus.
We observed that both MIG-17 and EMB-9 localize in the head-region to a pattern reminiscent of the extracellular matrix proximal to the pharynx bulb (Ihara et al., 2011). MIG-17 protein levels were detectable in larva stage one through larva stage 4, but became undetectable upon reaching the adult stage.