mNG::LIN-7, LIN-2::mK2 and mNG::LIN-10 were found to bebroadly expressed in the worm. In L3 larvae, they were prominentlyexpressed in VPCs and neurons, whereas only LIN-7 and LIN-10 weredetectable in the somatic gonad primordium. In the VPCs,endogenous mNG::LIN-7 was strongly cytosolic, frequently found atpunctae, and occasionally localized to basolateral membranes.
Expression of gcy-35 was consistently observed in URX, AQR, and PQR sensory neurons; gcy-35 expression was also observed in ALN, SDQ and BDU neurons and variably in AVM, PLM and PLN neurons, pharyngeal and body wall muscles, and the excretory cell.
mNG::LIN-7, LIN-2::mK2 and mNG::LIN-10 were found to bebroadly expressed in the worm. In L3 larvae, they were prominentlyexpressed in VPCs and neurons, whereas only LIN-7 and LIN-10 weredetectable in the somatic gonad primordium. In the VPCs,endogenous mNG::LIN-7 was strongly cytosolic, frequently found atpunctae, and occasionally localized to basolateral membranes.
The endogenous lin-28 promoter drives lin-28::GFP expression in neurons and hypodermis, where lin-28 is known to be expressed (Moss et al., 1997). Using spinning disk microscopy we also detected lin-28p::lin-28::GFP expression in Z1 and Z4 cells, which are precursors of somatic gonadal tissues.
lin-31p::cfp and LIN-12::GFP are expressed in multipotent VPCs during larval (L)2 stage and before induction in the L3 stage. lin-31p::cfp expression remains high in VPCs of dauer larvae. LIN-12::GFP remains expressed in VPCs in dauer larvae.