PRG-1 levels were reduced in L1/L2 and L2/L3 worms when compared with L4 worms, as well as young and gravid adults. The PRG-1 protein could be detected in embryo extracts, but not in the
glp-4(
bn2) mutant strain, suggesting that this protein is expressed in the germline. PRG-1 was also present in protein extracts from both female- and male-enriched populations. The expression of
prg-1 was reduced in wild-type worms cultured at 25 centigrades.