The
pry-1 reporter gene is widely expressed throughout development. Expression starts halfway through embryogenesis and is mainly localized to the ventral and lateral hypodermal cells. At the early L1 stage,
pry-1 is expressed at high levels in the lateral hypodermal cells (or seam cells) V5 and V6 and in the Q neuroblasts QL and QR.
pry-1 is also expressed in the ventral hypodermal (P) cells P7/8 to P11/12, body wall muscle cells, and neurons in the head, the tail, and the ventral nerve cord. No differences in
pry-1 expression levels between QL and QR was observed, but this may be a result of PRY-1 GFP overexpression. At the end of the L1 stage,
pry-1 is expressed at high levels in all seam cells. Expression was also observed in the QL and QR daughter cells. At later larval stages,
pry-1 is expressed at high levels throughout the animal, including hypodermal cells, body wall muscle cells, and many neurons in the ventral nerve cord and head and tail ganglia. In addition,
pry-1 is expressed in the vulva precursor (Pn.p) cells and in the developing vulva and male tail.