lit-1 is expressed in amphid sheath glia, among other cells. In addition, the expression pattern of this reporter partially overlaps with that of ptr-10, a gene expressed in ensheathing glia of other sensory organs.
PTR-18 and GRL-7 are temporally localized along the periphery of the apical membranes of hypodermal and P neuronal progenitor cells during late embryogenesis and are subsequently targeted to lysosomal degradation before hatching. PTR-18::GFP was first detected along the apical side of surface cells that cover the whole body, which consists of hypodermal, seam, and P cells at the 3-fold stage during embryogenesis. During the early time point, the majority of the 3-fold embryos initially showed apical localization of PTR-18::GFP. As development continued, the percentage of the apical localization decreased, the fraction that exhibited localization of PTR-18::GFP in the vesicular punctate structures increased, and eventually, most of the embryos did not show its expression. These observations indicate that PTR-18::GFP first localizes at the apical side of the hypodermal, seam, and P cells, subsequently accumulates in the vesicular structures, and eventually disappears before hatching. PTR-18::GFP was alsoexpressed in the descendants of P cells, rectal epithelial F, K, and U cells, and some seam cells in late L1 larvae.
ptr-4 is expressed in most but not all external epithelia, starting at the time period when precuticle is first synthesized, from embryonic to L4 stages.
Both transcriptional and translational reporters revealed that ptr-23 is co-expressed with osm-8 in the hypodermis. The localization os restricted to foci within specific areas of the hypodermis.
PTR-18 and GRL-7 are temporally localized along the periphery of the apical membranes of hypodermal and P neuronal progenitor cells during late embryogenesis and are subsequently targeted to lysosomal degradation before hatching. Similar to PTR-18::GFP, GRL-7::mCherry::3xFLAG was first detected in the 3-fold embryos GRL-7::mCherry::3xFLAG localized along the apical side of the surface cells that cover the whole body as well as in the intracellular structures of these cells. As observed for PTR-18::GFP, the majority of the GRL-7::mCherry::3xFLAG embryos initially showed an apical distribution. However, as the embryos neared hatching, internal, vesicular localization became predominant. GRL-7::mCherry exhibits striped patterns of localization, which likely shows annular furrows.