Pser-2::mCherry reporter was expressed in head muscles, as well as several neurons in the head and ventral cord. The
ser-2 reporter was expressed in the first and second row of muscle cells, which are distinct from neck muscles (third and fourth row of muscle cells). The
ser-2 reporter was not expressed in interneurons that control locomotion, as expression did not overlap with Pglr-1::GFP and Plgc-55::GFP reporters that are expressed in the locomotion command neurons (unpublished data).
ser-2 reporter expression was also observed in 13 cells in the ventral cord that send commissures to the dorsal cord. Coexpression analysis with GFP reporters that specifically label cholinergic or GABAergic neurons showed that Pser-2::mCherry was highly expressed in a subset of GABAergic motor neurons, specifically the VD motor neurons.