Punctate immunoreactivity in the ventral and dorsal nerve cords and the nerve ring. The antibodies failed to detect UNC-11 in motor neuron commissures or in the dendrites of sensory neurons in the head. UNC-11 expressed in the coelomocytes and diffusively stained the intestine.
Similar to UNC-11 antibody staining, UNC-11::GFP expression appeared pan-neuronal and was also observed within coelomocytes. Not described by Nonet el al 1999, we observed GFP puncta in apical epithelial tissue just below the cuticle surface in both the head and in the body, an accumulation of UNC-11::GFP at the plasma membrane in the seam cells and weak staining in the vulval precursor cells (VPCs) at the interface between the VPCs and the vulval lumen. Finally, Nonet et al. described diffuse staining within the intestine whereas we did not observe detectable levels of intestinal expression, punctate or otherwise.
At the time of invasion GFP::UNC-60A was present throughout the uterine tissue and at elevated levels in the anchor cell (1.35-fold greater levels versus neighboring uterine cells; n = 11 animals examined). GFP::UNC-60A was also more enriched at the invasive cell membrane (1.29-fold enrichment vs. 0.75-fold for neighboring uterine cells; n = 11 animals examined).