Examination of the ALG-2 expression during larval development did not reveal differences in expression during the four larval stages and adults. During embryogenesis ALG-2 started to be expressed from pre-morphogenetic embryonic stages.
ALG-1 and ALG-2 were expressed in most somatic cells but exhibited some tissue specificity in the head region. Pharyngeal cells predominantly expressed GFP::ALG-1, while certain head neurons adjacent to the pharynx were enriched for RFP::ALG-2. Consistent with the Western blot results, there was a global decline in expression of GFP:: ALG-1 but not RFP::ALG-2 as the animals aged.
ALG-1 and ALG-2 were expressed in most somatic cells but exhibited some tissue specificity in the head region. Pharyngeal cells predominantly expressed GFP::ALG-1, while certain head neurons adjacent to the pharynx were enriched for RFP::ALG-2. Consistent with the Western blot results, there was a global decline in expression of GFP:: ALG-1 but not RFP::ALG-2 as the animals aged.
Expressed in the cytoplasm from early embryogenesis to adulthood in most, if not all, cells in C. elegans. There is no clear difference in the expression patterns of alg-1 and alg-2.
Expressed in the cytoplasm from early embryogenesis to adulthood in most, if not all, cells in C. elegans. There is no clear difference in the expression patterns of alg-1 and alg-2.
While ALG-2 levels remained relatively constant from the fourth larval stage (L4) through day 11 of adulthood, ALG-1 levels decreased precipitously during adulthood.
While ALG-2 levels remained relatively constant from the fourth larval stage (L4) through day 11 of adulthood, ALG-1 levels decreased precipitously during adulthood.
By immunostaining of extruded gonads using a newly generated ALG-1-specific antibody, we observed that ALG-1 is localized to the DTC of the wild-type gonads but not in alg-1(gk214).