EMB-9::mcherry strongly localized to BM in pharynx. We also confirmed strong expression of EMB-9::mcherry in coelomocytes, but not in the strain expressing mkate2::NID-1. The mkate2::NID-1 and EMB-9::mcherry are similarly localized to BM at gonad and uterus.
Consistent with previous studies, the type-IV collagen α1 chain construct, EMB-9::mCherry, when expressed from the integrated transgene tkTi1[emb-9::mCherry], localized to the gonadal basement membrane in WT C. elegans.
Both MIG-17 and EMB-9 localize in the head-region to a pattern reminiscent of the extracellular matrix proximal to the pharynx bulb. EMB-9 protein levels increased as animals progress through the larval stages, achieving maximal expression in the adult stage.
To determine whether the APC might function at GABA synapses, we analyzed the subcellular localization of a GFP-tagged APC subunit EMB-27 CDC16 (GFP::EMB-27) relative to mCherry-tagged SNB-1 Synaptobrevin (mCherry::SNB-1), which marks presynaptic sites, in GABA neurons (Fig. 7A). We found that approximately 90% of the GFP::EMB-27 puncta co-localized with mCherry::SNB-1 puncta (90.28 ± 14.11%, n = 20 animals) and vice versa (90.11 ± 15.67%, n = 20 animals).