This transcriptional
gap-3 reporter was expressed throughout C. elegans development and first detected in ball stage embryos. At the 1.5-fold stage, the GFP signal was observed mainly in hypodermal cells, and expression persisted throughout larval development until adulthood. Furthermore, the
gap-3 reporter was expressed in some head neurons, in the spermatheca, in the seam cells, in the body wall muscles, and in the sex muscles of the adult hermaphrodite. Expression was also observed in some uterine cells but authors did not detect a GFP signal in the vulval cells, the excretory duct cell or the P12.p cell at any developmental stage.