GFP-tagged synaptojanin was localized to puncta at neuromuscular junctions in the ventral nerve cord of unc-26 animals (genotype oxIs92 II; unc-26(s1710) IV).
Consistent with previous studies, the type-IV collagen α1 chain construct, EMB-9::mCherry, when expressed from the integrated transgene tkTi1[emb-9::mCherry], localized to the gonadal basement membrane in WT C. elegans.
Type IV collagen localizes to the BM and is expressed primarily in the body wall muscles, with the neuronal ring ganglion, spermatheca, uterine mucles, vulval muscles, and the distal tip cell of the somatic gonad also contributing collagen during specific larval stages.
A gradual increase in the expression level of CPTA during the development from embryo through larva L1-2 and L3-4 to adults was clearly evident. The relative intensities of the CPTA-specific product with respect to actin IV were 2.7, 2.7, 7.0 and 7.8 for the eggs, L1-2, L3-4 and mixed stage animals respectively.
As expected, localization of mkate2::HIM-4 to the pharyngeal basement membrane was observed at the L1 stage. In contrast to the uniform localization on the basement membrane of type IV collagen or nidogen, which are major components of the basement membrane, that of mkate2::HIM-4 was polarized at the anterior part of the pharyngeal basement membrane. A unique polarized localization pattern was also observed in the pharyngeal basement membrane at stage L4. These observations indicate that polarized HIM-4 localization in the anterior part of the pharyngeal BM is maintained throughout the L1-L4 stages. The localization was also observed at the rachis during gonadal development at the L4 stage; this observation was consistent with that of previous reports.