beta-gal staining detected in all developmental stages including embryos and is most intense in early larval stages. Although NLS was present in the construct between the promoter sequences and lacZ gene, beta-gal staining was found in the cytoplasm and at the membrane.
mrp-1 expression is observed in cells of the pharynx, the pharynx-intestinal valve cells and the anterior intestinal cells, the epithelial cells of the vulva and the rectum-intestinal valve cells. Similar tissue distribution is observed with
mrp-1::gfp. In L3, staining seen in pharynx, pharynx-intestinal valve and first intestinal cells, intestinal-rectum valve cells. In young adults, staining is detected in epithelial cells of the vulva.