At approximately 240 min post-fertilization, UNC-39::GFP appeared in more posteriorly located nuclei in addition to the anterior nuclei. Posterior nuclei expressing
unc-39::gfp included Z1 and Z4, the M cell, and the embryonic coelomocytes. Occasionally expressed in additional posteriorly located cells. Expression in M and the coelomocytes was transient and no longer observed by 600 min post-fertilization, whereas Z1 and Z4 expression persisted into the L1 larval stage.