APX-1 protein is detected first in the late 2-cell stage at the anterior periphery of P1. It is found in and EMS at the 4-cell stage. Later, it is seen faintly in P3.
The lin-11 gene is specifically expressed in ventral cord motor neurons and thus the Plin-11::gfp reporter specifically labels the ventral cord cells, P3.aap-P8.aap.
GFP expression in most if not all nuclei during embryogenesis and in newly hatched L1s. During larval development, fluorescence observed in many nuclei in the head and tail regions. GFP was also present in hypodermal cells throughout development. At the time of vulval induction, GFP was visible in all P3.p to P8.p cells and persisted until after the P3.p to P8.p cell divisions and vulval morphogenesis were complete.
LIN-39 protein levels were not uniform among vulval precursor cells. P3.p and P4.p displayed the lowest LIN-39 levels, and the level of LIN-39 in P4.p was not significantly higher than that in P3.p. Surprisingly, the LIN-39 level was the highest in P5.p and decreased from P5.p to P8.p. At a later developmental time (late L2 larvae), as previously reported, the LIN-39 peak was found on P6.p.
At 1 to 8-cell stage, ubiquitous with stronger staining in P2 or P3. Ubiquitous at 10-cell through bean embryo. Staining in gut, Z2, Z3 and unidentified anterior cells at comma through pretzel stages.
Analysis of wild-type worms showed that eff-1p::GFP was expressed in the daughter cells of P3.p, P4.p, and P8.p that are committed to fusion, and was absent in the daughter cells of P(5-7).p which escape the fusion fate.
Before vulval induction occurs, lin-39 was expressed uniformly in the VPCs. At the time of vulval induction, lin-39 expression increased dramatically in P6.p. P3.p, P4.p, and P8.p showed the lowest levels of lin-39 expression.