Like in the pharynx, PEB-1 was first detected in the hypodermal nuclei in comma stage embryos (approximately 400 min) and remained detectable until hatching. In 1 1/2-fold stage embryos, the PEB-1 protein was detected in the nuclei of most, if not all, hypodermal cells including
hyp5,
hyp6, and
hyp7 and the H, P, and V cells. After hatching, PEB-1 remained detectable in most hypodermal cells although it decreased in later larvae and was undetectable in adults. In the L1 lateral hypodermis, the PEB-1 protein was detected in H0, H1, H2, V1 to V6, and T, and their anterior and posterior daughters, as well as in the nuclei of the
hyp7 syncytium. PEB-1 was also detected in many of the dorsal and ventral hypodermal nuclei in the head and the ventral hypodermal nuclei in the tail. Notably, PEB-1 was not detected in larval P cells. In addition, PEB-1 was not detected in other neuroblasts including Q and T.p.