- Expr3009
Expressed in ALA, AVG, PHB, PDA, PVW, RIC, SAA. Faint or variable expression observed in RMDV/SMDV, PHA). No male specific expression.
- Expr3021
Expressed in AIM, ASG, AVA, AVG, AVL, CEP, PVD, PVW, RIC/AIZ, RIV, SMD, URA,
uv1. Male specific expression in 6 out of 9 CP.
- Expr3004
Expressed in AIA, RID, PVW, I5, MC. Faint or variable expression observed in ASI, M4, head muscles and an extra pair of cells in the head. No male specific expression.
- Expr8675
Expression in the nervous system: ADE, AIY, ALM, ALN, AVA, AVK, AVM, BDU, CAN, DAn, DVA, DVB, DVC, FLP, HSN, LUA, PLM, PLN, PVC, PVM, PVP, PVQ, PVT, PVW, RID, RIS, SDQ, URB, MC.
- Expr11372
ADL (100%), ADF (100%), ASH (92%), RIC (88%), I2 (59%), PVR (35%), PVQ (72%), PVW (72%), HOB (25%), ray neurons (50%), protractor muscles (31%), intestine (100%), head muscle (6%). Percentages correspond to the fraction of animals examined that expressed GFP in the indicated neurons. Above 50% marked as certain, below as uncertain.
- Expr11374
PVR (50%), PVQ (58%) PVW (58%), ADL (96%), ADF (72%), SMD (96%), RME (66%), AIA (64%), ASH (60%), AVG (56%), RIF (44%), PVS/PVU (17%), SPC (39%), oblique muscles (53%), intestine (90%). Percentages correspond to the fraction of animals examined that expressed GFP in the indicated neurons. Above 50% marked as certain, below as uncertain.
- Expr3237
Native
tor-2 gene expression is limited to just a few cells, including vulva muscles, a single cholinergic pharyngeal neuron (M1), two sensory neurons (AW class) and two interneurons (AVE) in the head, and a few neurons toward the tail, including a pair of PVW neurons. Importantly for this study,
tor-2 gene expression is unequivocally absent in DA neurons at any larval or adult stages.
- Expr8774
GFP fluorescence was detected in 20 neurons. In the head, DKF-2BGFP accumulated in amphid sensory neurons (ASE, AFD, ASG, AWC, and AWA), integrative interneurons (AIM, RIC, AIY, AVF, AVJ, AVK, ADA, and RMG), neurons involved in social feeding and O2 sensing (AUA, URX, PQR, AQR, and BAG), and the neurosecretory motor neuron NSM. DKF-2BGFP also accumulated in PVQ, PVR, and PVW interneurons in the tail. DKF-2BGFP was observed in late embryos, L1 to L4 larvae, and adult animals.
- Expr10592
Transcriptional reporters were expressed in neurons and body wall muscle and were similarly expressed in both males and hermaphrodites. Colocalization with other reporters and anatomical criteria enabled identification of the expressing neurons as the ciliated sensory neurons OLL, PHA and PQR, the nonciliated sensory neurons URY and URX, the touch receptor neurons ALM, PLM, AVM and PVM, the interneurons in the retro-vesicular ganglion RIF and AVF, the command interneurons AVD and PVC, the ring motor neurons RMED and RMEV, and two other neurons tentatively identified as either PVQ or PVW and DB2. No expression was observed in amphid or male-specific neurons.
- Expr1839
During embryogenesis
kal-1 expression is first detectable at about the 160- to 200-cell stage in a group of eight to ten neuroblast descendants of the AB blastomere. At no stage during embryogenesis were
kal-1 driven reporters detectably expressed by epidermal cells. After elongation of the embryo is completed, the expressing cells have roughly reached the positions they will occupy in larval stages. During post-embryonic development, the expressing neurons remain largely the same, except for the recruitment of some sexually dimorphic neurons. Three groups of neurons express the
kal-1 constructs post-embryonically. One group is located in the anterior ganglia. Here, about 15 neurons express the gene. They include some interneurons and some sensory neurons. A second group is located at mid body and is composed of the two canal associated neurons (CANs) and, in adult hermaphrodites, of the hermaphrodite specific neurons (HSN). The third group is located in the tail region, where three to six neurons express the construct. Among these are the PDB neuron and another interneuron, possibly PVW. Two male-specific neurons were also identified, the interneu on EF3 and one of the neurons of sensory ray 5. As during ventral enclosure, also during male tail formation,
kal-1 is not expressed by epithelial cells but by neurons.