Fluorescence of the mCherry driven from the recombineered
sir-2.1 fusion gene was observed in nerve cells of the head, the ventral nerve cord and the tail, and in the hypodermis, as described previously for a conventional reporter gene fusion (Wang and Tissenbaum, 2006). On top of this, however, mCherry fluorescence was also observed in muscle and intestinal cells, and potentially other additional cell types.