A stably integrated SPR-4::GFP construct under the control of the endogenous spr-4 promoter is expressed predominantly in neurons in adult worms, as indicated by colocalization with the neuronal marker prab-3::mCherry.
The spr-3 transcript is expressed in all stages but at different levels. The message is present in high amounts in eggs, L2, and adult stages, but more weakly expressed in the L1, L3 and L4 stages.
Asymmetric expression of imb-2 in AWCON was also observed in our initial assessment of the imb-2 expression pattern using imb-2 fosmid::GFP in which we fused GFP with the C terminus of IMB-2 by homologous recombination, that rescued imb-2(vy10) mutants.