The transgenic animals displayed mCherry in all five pairs of gonadal sheath cells. However, fluorescence in Sh5 was only seen in a subset of the adult animals, suggesting lower endogenous
lim-7 expression . As expected, the URA neurons also expressed
lim-7(+)::mCherry. Moreover, at least 10 additional cells, which appear to be neuronal, expressed
lim-7(+)::mCherry near the isthmus and terminal bulb of the pharynx in a similar region to the URA neurons. Late embryos also appear to express
lim-7(+)::mCherry in the same region. The exact identity of these cells requires further confirmation as many different neurons reside in this region in addition to other cell types.