Expr3049
Expression from both extrachromosomal arrays and chromosomal integrants was observed, and the results were fully consistent. LIN-18::GFP was expressed in neurons, body wall muscle, and vulval tissue. In the vulva, LIN-18::GFP was expressed in P5.p, P6.p, and P7.p and all their descendants throughout the L3 and L4 stages. In most (>90%) L4 stage individuals, descendants of P5.p and P7.p expressed higher levels of LIN-18::GFP than descendants of P6.p. No obvious difference in expression levels was found among different descendants of a single VPC (e.g., between P7.paa, P7.pap, P7.ppa, and P7.ppp).