Expr13641
The W01A8.1 translational fusion constructs resulted in high levels of cytoplasmic proteins present in intestinal and epidermal cells on vesicular structures with the characteristic appearance of lipid droplets. This pattern of expression and cellular distribution was observed beginning at the three-fold embryonic stage and continued throughout development to adulthood. To confirm that the observed GFP-associated vesicular structures were indeed lipid droplets, transgenic animals were stained with the lipophilic reagent LipidTox as previously described (O'Rourke et al., 2009). The translational GFP fusion protein reporters were localized at the periphery of fat droplets that were LipidTox positive.