wdr-5.1 was nuclear-localized in Y/PDA throughout transdifferentiation.In some cases faint additional WDR-5.1::GFP staining was observed in the cytoplasm.
WDR-46::GFP was first visible in late-stage embryos as multiple distinct puncta. In L4 larvae and young adults, WDR-46::GFP fluorescence occurred as puncta in the hypo- dermis and intestine. We also observed WDR-46:: GFP in the body wall muscle, germline, and several cells in the head.
Tissues with consistent wdr-23a and wdr-23b GFP expression in three independent lines included the intestine, hypodermis, and muscle. GFP was also observed in other less-recognizable cells in the head and tail.
Confocal microscopy showed that either FAT-5::GFP or FAT-7::GFP could colocalize with both CYTB- 5.1::FLAG::mCHERRY and CYTB-5.2::FLAG::mCHERRY, which express in the intestine of worms similar as CYTB-5.1::GFP and CYTB-5.2::GFP.