aptf-1 was expressed strongly during late embryogenesis and early larval development in three types of interneurons, the paired neurons AIB and RIB and the unpaired neuron RIS. The expression in RIS was the strongest. Expression after embryonic development decreased until the adult stage. Expression of a GFP-tagged fosmid confirmed the expression in these neurons and showed that APTF-1::GFP was present until the adult stage.
In wild-type worms, relative asfl-1 mRNA expression levels were high in embryos (16-fold), increased dramatically at the L4 (10-fold) and young adult stages (30-fold), indicating that asfl-1 expression is developmentally regulated.
We found that blmp-1 transcription is detected in the tail-spike cell as early as the mKatePH reporter (1.5-fold stage). blmp-1 transcription continues until the tail-spike cell dies with a characteristic rounded refractile morphology at the 3.7-fold stage.
Moderate bwm, mosaic, tail hyps, earlies bwm is comma with 1-2 cells +, by 3-fold fairly strong in many bwm; early gut at 12E, strong gut at 1.5 fold then fades to cytoplasmic and gone.
Until the comma stage die-1 is expressed in ASER and ASEL (fig 2). Asymmetric die-1 expression becomes apparent at the 3-fold stage of embryogenesis in ASEL.