glp-1(2in_493) is expressed in several neuronal precursor including the P blast cells in embryos at the comma stage. After hatching,
glp-1(2in_493) expression was detectable in various neurons throughout all larval stages. At the L1-L2 stages, GFP fluorescence was evident in the ventral nerve cord. During the L3-L4 stages,
glp-1(2in_493) expression was present in most, if not all, ventral nerve cord neurons. In addition, several neurons in the head and tail regions expressed
glp-1(2in_493). For example, most cells of the pharyngeal nerve ring, including certain amphid neurons such as ASK, ADL, ASI and ASH, displayed
glp-1(2in_493) expression. Some neuronal cells from the tail, including the phasmid neuron PHB, were also glowing.