The
ttll-11b reporter was exclusively expressed in ciliated extracellular vesicle-releasing neurons (EVNs). Expression of GFP driven by the
ttll-11b promoter was observed in the inner labial type 2 (IL2) ciliated sensory neurons in both males and hermaphrodites, as well as the male-specific PKD-2-expressing neurons (CEMs in the head; HOB and RnBs in the tail). The IL2, CEM, HOB, and RnB neurons comprise the EVNs, which release bioactive EVs to the environment.