GFP was observed in nuclei of a few neurons in the head, probably in the nerve ring, and 1 or 2 neurons in the tail. The GFP expression was only seen in the L1 and was extremely faint, much weaker than seen in strains, such as UL3509 or UL3404, transgenic for reporter gene fusions with gfp inserted immediately after the
daf-19a/b or
daf-19c start codon.