Expr11202

Expressed in AWC and ASE (Fig. 2B).

Expr15700

daf-2b is present at all life stages and daf-2a is between 4 and 7 times more abundant than daf-2b.

Expr11201

Expressed in AWB, AWC, AFD, ASE, and ASI (Fig. 2B).

Expr16016

Endogenously-tagged short mec-2B isoform is also detectable in touch neurons, with a similar punctate expression. In agreement with our transgenic MEC-2B::GFP overexpression results, we find endogenous short isoform MEC-2B::RFP expressed abundantly in many head and tail neurons in addition to touch neurons.

Expr15702

Using the daf-2b splicing reporter in dauer larvae, we observed expression only in the nervous system.

Expr11610

The wild-type sygl-1 promoter drove expression of Histone 2B::GFP in the distal-most germ cells.

Expr15701

The daf-2b splicing reporter was highly expressed in the hypodermis and intestine of L1 and L2 larvae.

Expr16015

The mec-2B (short) translational reporter revealed expression in touch neurons as well as many additional neurons in the head and tail. This corroborates our RNA-seq observations that the non-canonical short mec-2B isoform is the predominant form of mec-2 expressed in wild-type animals, with expression in both touch neurons and additional neuron types. To test whether the short mec-2B isoform is expressed in specific amphid neurons, we labeled the amphid AWB and AWC olfactory neurons with an odr- 1p::RFP reporter. MEC-2B::GFP expression is clearly visible in both AWB and AWC neuronal cell bodies, as well as their axons and dendrites.

Expr13413

Strong pkc-2B promoter activity was evident in intestine and muscle. B promoter-dependent transcription was also detected in AFD and AWC.

Expr7896

Expression of asd-2a::GFP was detected in hypodermis and pharyngeal muscles. Expression of asd-2b::RFP was observed in the body wall muscles and the pharyngeal muscles.