Expr11590

LIN-12::GFP is present and membrane-localized in all SM sub-lineage cells from the 2-SM stage through the 8-SM stage. At the 16-SM stage the expression of LIN-12::GFP becomes restricted to the four SM descendants that are fated to become VM2s. As these cells begin to differentiate into VM2s, LIN-12::GFP becomes nuclear localized. Thus LIN-12 is present and activated in cells fated to become VM2s. LIN-12 is present in M.vlpaaap, M.vlpaapa, M.vrpaaap and M.vrpaapa cells.

Expr13754

R1-1.3 kb::Δpes-10::gfp: SM cells, Vulval muscles, HSNs, Unidentified head cells

Expr13403

sem-4::GFP is expressed throughout the M lineage from the 1-M stage to the 18-M stage, and in the SM sub-lineage from the 2-SM stage to the 16-SM stage, before the cells differentiate. When these cells terminally differentiate, sem-4::GFP becomes undetectable in the differentiated coelomocytes and vulval muscles, but remains expressed in the differentiated BWMs.

Expr13749

R1::Deltapes-10::gfp was expressed in SM cells, vulval muscles, vulval cells, HSN neurons, and unidentified head cells.

Expr9973

Expression of gfp::sem-2 was first detectable in a subset of cells of the E and MS lineages in early gastrulating-stage embryos. The gfp::sem-2 expression persisted through embryonic and larval development in many cell types, including vulval, hypodermal and intestinal cells. gfp::sem-2 expression in the M lineage was first detectable at the 16-M stage in the SM mother cells, M.v(l/r)pa, and remained in both of their daughter cells, M.v(l/r)paa and M.v(l/r)pap. The presence of GFP::SEM-2 in M.v(l/r)pap was transient: GFP::SEM-2 was not detectable after M.v(l/r)pap differentiated into BWMs. However, GFP::SEM-2 persisted in the nuclei of the SM cells and all their descendants until the 8-SM stage, and became undetectable at the 16-SM stage.

Expr10032

Single molecule fluorescence in situ hybridization (SM- FISH) was used to show that mec-17 is indeed highly expressed in the TRNs; the PLM neurons of WT animals had 60 +- 5 mec-17 messenger RNA molecules.

Expr11254

cwn-1 is predominantly expressed in the posterior body wall muscle (BWM) and in the M cell/SM lineage. On average, the wild-type SMs each express 50 transcripts of cwn-1 prior to the polarity choice of P7.p.

Expr13745

Consistent with previously reported bed-3::gfp reporter expression, bed-3 mRNA (smFISH) was predominantly expressed in hypodermal cells and vulval cells. We next examined whether bed-3 is expressed earlier in the M lineage using ayIs6[hlh-8::gfp]. We found that bed-3 is expressed in M daughters and descendants as well as SM cells. In L1 and L2 stages, the bed-3 smFISH signal appeared stronger in 2 M and 4 M stages compared to 8 M, 16 M and 18 M stages. In the L3 stage, bed-3 is expressed in SM cells near the vulval region of the animal.

Expr1433

VAB-8L was expressed in AVKR, and VAB-8S was expressed in the CANs. Other cells expressing VAB-8 included motorneurons in the ventral nerve cord that extend posteriorly directed processes(DBs and DDs), interneurons that extend posteriorly directed axons in the dorsal lateral and ventral lateral nerve bundles (SI and SM classes), and additional unidentified neurons in the pharynx and head. VAB-8 was also expressed in body wall muscle.

Expr606

Immuno fluorescence staining with antisera to ceTwist indicated similar expression pattern to reporter fusion. CeTwist first detected in L1 in defecation-associated muscles and in small number of neuron-like cells in the head. In later larvae, CeTwist was detected in SMs and their descendants. Expression in M and its descendants prior to to the SM stage was detected with reporter fusion but not with antibody. Expression in mature coelomocytes was also only detected with reporter fusions.