MIG-13::GFP fluorescence was not observed in QL.x. However, MIG-13::GFP is expressed in the migrating QR.x, and its GFP fluorescence is present on the cell surface. Time-lapse imaging revealed dynamic changes of
mig-13 expression during migration: MIG-13::GFP was detectable at the initial stage of QR.x migration; MIG-13::GFP gradually increased its fluorescence during migration and reached its maximum level at the end of migration. MIG-13::GFP fluorescence in QR.a/ap is stronger than that observed in QR.p/pa. The presence of MIG-13::GFP in QR.x was confirmed using an independent transgenic integrant (muIs42).