The
mec-2B (short) translational reporter revealed expression in touch neurons as well as many additional neurons in the head and tail. This corroborates our RNA-seq observations that the non-canonical short
mec-2B isoform is the predominant form of
mec-2 expressed in wild-type animals, with expression in both touch neurons and additional neuron types. To test whether the short
mec-2B isoform is expressed in specific amphid neurons, we labeled the amphid AWB and AWC olfactory neurons with an odr- 1p::RFP reporter. MEC-2B::GFP expression is clearly visible in both AWB and AWC neuronal cell bodies, as well as their axons and dendrites.