We examined the expression and localization of endogenous NEKL-4 by using CRISPR/Cas9 to engineer
nekl-4::mneongreen and
nekl-4::mscarlet strains. These CRISPR-generated reporters use the
nekl-4 locus as well as a short flexible linker, and have significantly dimmer fluorescence than the extrachromosomal
nekl-4p::
nekl-4::gfp array [Expr15275]. With both extrachromosomal and CRISPR reporters, NEKL-4 was observed in the same neurons and similar subcellular locations.