We observed that, in WT animals, ATG-9 endogenously tagged with GFP (ATG-9::GFP) is strongly expressed in the nerve ring , as well as in the somatic sheath and spermatheca. We also found strong expression of ATG-9::GFP in the hypodermis, a tissue that contains abundant LDs detectable by BODIPY 493 staining.
ATG-16.1::GFP was diffusely expressed in the cytoplasm in most, if not all, cells during embryogenesis. At post-embryonic stages, ATG-16.1::GFP was widely expressed, including in neurons, pharyngeal muscles, body wall muscle cells and intestinal cells.
ATG-16.2::GFP was diffusely expressed in the cytoplasm in most, if not all, cells during embryogenesis. At post-embryonic stages, ATG-16.2::GFP was widely expressed, including in neurons, pharyngeal muscles, body wall muscle cells and intestinal cells.
A 64-bp fragment, located between 366 and 303 bp upstream of the egl-17 ATG, was sufficient to activate reporter expression in vulD and vulC cells from mid-L4 through adulthood, termed the vulDC enhancer.
A minimal region of 116 bp (positioned at - 200 to -84 bp upstream of the trx-1b isoform ATG codon) drives GFP expression in ASJ, thus containing the putative cis-regulatory motif responsible for ASJ expression.
pJW5 contains a 1.3-kb fragment located between 5.1 and 6.4 kb upstream of the lin-39 ATG that drives GFP expression in P6.p at the time of vulval induction and in the sex myoblasts descendants.