lsy-27 is expressed very broadly throughout the embryo. Expression can already be observed in one-cell embryos and continues to about the comma stage, when expression starts to fade out. By the comma stage, most neurons, including ASEL/ASER, have terminally divided and begun to terminally differentiate. No expression is observed after hatching in larvae or in adult animals. Through colocalizing expression of the
lsy-27 reporter with an ASE-specific mCherry reporter, we confirmed that
lsy-27 is expressed in both ASE neurons in the comma-stage embryo when ASE laterality is established.