cyp-8 protein as expression of beta-galactosidase was localized specifically in the gut cell nuclei. Expression is detected at all stages from embryo through to adult. Promoter region of Ce-CYP-8 examined with downstream coding sequence either including or excluding the NLS containing tail domain of
cyp-8. This shows that non-cyclophilin tail domain directs the localization of this enzyme to the gut cell nuclei. Constructs containing promoter cyclophilin domain and tail domain exhibited nuclear localized gut staining in a non-nuclear localized vector. Animals transformed with the promoter and cyclophilin domain but lacking the NLS-rich tail domain show diffuse gut staining extending to the pharynx.