At the four-cell stage, HMR-1 accumulation was asymmetric as there was less HMR-1::GFP intensity at the EMS-P2 contact in HMR-1::GFP-expressing embryos.
A full-length hmr-1::gfp transgene driven by the endogenous promoter localizes to epidermal adherens junctions and was able to rescue hmr-1(zu389) homozygotes to viability.
MPK-1A was present in both GL+ and GL- animals, but MPK-1B protein was found only in GL+ animals. Thus mpk-1b RNA and its MPK-1B protein are predominantly expressed in the germline.
A HMR-1::GFP reporter which shows comparable localization patterns to the endogenous HMR-1 is strongly enriched at the interface between the basal pharyngeal surface and neuroectoderm during involution in a supracellular patch. This signal is distinct from the known localization at the apical side of the pharynx, which is dorsal to this patch.
SKN-1B::GFP is expressed in ASI neurons. DiI staining confirms SKN- 1B::GFP in the ASI neurons and identified two of these additional neurons as being the ADLs.
TTM-1B::GFP was expressed in multiple tissues including intestine, head neurons, seam cells, hypodermis, and the vulva consistent with the tissue distribution pattern displayed by Pttm-1b::GFP transgenic animals.